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Intracytoplasmic Sperm Injection (ICSI) is an assisted reproduction technique included within in vitro fertilisation (IVF) treatment. It has enabled pregnancy to be achieved successfully in couples diagnosed with severe male factor infertility. The man must provide a semen sample or must undergo a testicular biopsy – if necessary – in order to extract and select the best spermatozoa which will be used to fertilise the oocytes.



  • Men with a low sperm count, sperm motility problems or poor sperm morphology.
  • Men who have had a vasectomy.
  • Infectious diseases or infertility caused by immune factors.
  • Difficulty ejaculating normally, as in the case of retrograde ejaculation (neurological problems, diabetes, etc.).
  • Cases of cryopreserved samples from men who have had a vasectomy or have had chemotherapy or radiotherapy. These samples are very valuable as there is a limited quantity and ICSI allows their use to be optimised.
  • Other factors: repeated failure following several cycles of IVF, a low number of oocytes obtained from follicular puncture, or when it is necessary to identify healthy embryos in the case of pre-implantation genetic diagnosis (PGD).


90% of patients who undergo an assisted reproduction treatment at IVI become pregnant.


IVI is a pioneer in the latest assisted reproduction technology in order to present the best results.


97% of our patients recommend IVI.
IVI provides personalised care and support during all stages of treatment.


We are not the most expensive choice. We offer the most treatment options in order to achieve the best results.



Intracytoplasmic Sperm Injection allows the egg and the sperm to be brought together directly, facilitating fertilisation. A sperm that has low motility or poor morphology would have greater difficulty achieving this naturally or through the technique of classic IVF.


Personalised assessment of patients for determining the most suitable fertility treatment.

1. Ovarian stimulation

Ovarian stimulation during IVF consists of the administration of daily injections which cause the ovaries, instead of producing a single ovum which is what they do naturally each month, to produce more oocytes so that a larger number of embryos can be obtained. This treatment takes place at IVI assisted reproduction clinics and lasts between 10 to 20 days, depending on the protocol used and the speed of response of each patient. During treatment, a series of 3 or 4 ultrasound scans are carried out and the amount of estradiol in the blood is determined to check that there is normal growth and development of the follicles.

2. Follicular puncture and the laboratory

When we have evidence through the ultrasound scans that the follicles have reached an adequate size, and we consider that there are a suitable number of oocytes, we schedule follicular puncture 36 hours after administering an injection of the hormone hCG which causes the oocytes to mature in a similar way to the way they would in a natural cycle. Puncture is carried out in the operating theatre under sedation so that the patient does not feel any kind of discomfort during the procedure, which lasts approximately 15 minutes.


Once we have the oocytes after follicular puncture, and the spermatozoa with the best motility and morphology have been selected, we proceed to microinjection of the oocyte. During the process of ICSI, the selected sperm is placed in a tiny pipette and is injected directly into the ovum. In this way, we facilitate fertilisation to the greatest possible extent. The embryos obtained are allowed to develop for 3 to 5 days in the laboratory before being transferred into the maternal uterus once again.

Embryo culture in the laboratory

The resulting embryos are observed in the laboratory day after day and are classified according to their morphology and their ability to divide. Some embryos can become blocked in their development, and these will be discarded as they are considered to be non-viable.

3. Transfer

Transfer involves inserting the best embryos into the mother’s uterus with the help of a specially designed cannula. The procedure is carried out in an operating theatre, although in this case it is not necessary to use sedation since it is a quick and painless procedure.

Vitrification of remaining embryons

Once the embryo transfer has been made, we vitrify the remaining good quality embryos so that they can be used in a later cycle without the need for further ovarian stimulation.


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