Intracytoplasmic Sperm Injection (ICSI) is recommended in cases of male infertility, thus enabling fertilization. This procedure consists of extracting a spermatozoon from a sample of semen or by testicular biopsy to select the most appropriate sperm.

What is ICSI?

What is ICSI?

In what cases is it indicated?

  • Men with a low sperm count, sperm motility problems or poor sperm morphology.
  • Other factors: repeated failure after several cycles of IVF and AI, low number of eggs after pick-up, etc.
  • History of fertilization failure.
  • Men who have had a vasectomy.
  • Assisted reproduction treatments in which a Preimplantation Genetic Test (PGT) is performed.
  • Infectious diseases or infertility caused by immune factors.
  • Cases of particularly valuable semen samples due to their limited quantity, as the ICSI technique optimizes their use:
    • Men undergoing radio or chemotherapy treatment.
    • Patients with difficulty achieving normal ejaculation, as in retrograde ejaculation.
    • Men with no sperm in the ejaculate, in which case sperm can be obtained through testicular biopsy.


90% of patients who undergo an assisted reproduction treatment at IVI become pregnant.


97% of our patients recommend IVI.
IVI provides personalised care and support during all stages of treatment.


IVI is a pioneer in the latest assisted reproduction technology in order to present the best results.


We are not the most expensive choice. We offer the most treatment options in order to achieve the best results.




To perform the ICSI it is necessary to perform controlled ovarian stimulation on the patient or the donor (in the event donated eggs are used), in order to obtain a good number of eggs through follicular puncture.

In turn, the semen sample is obtained (or thawed if donor semen is used) and it is prepared. The preparation consists of processing the sample to obtain a fraction with the best sperm and also to activate its fertilizing capacity.

Once we have both gametes, the embryologist proceeds to perform the ICSI. To do this, the samples are placed on a culture plate and, using a microscope and micromanipulators, a sperm is selected, immobilized and inserted inside the egg using a pipette. This procedure is repeated with each of the mature eggs obtained. This way, we maximize fertilization.

The next day, the embryologist evaluates the microinjected eggs to see which of them have fertilized correctly (called zygotes). The zygotes are kept in culture and produce embryos, which are evaluated every day until their development is complete. At that time, the best embryos are selected: one is transferred to the mother’s womb and the rest are vitrified for possible later use.

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