- Men with a low sperm count, sperm motility problems or poor sperm morphology.
- Men who have had a vasectomy.
- Infectious diseases or infertility caused by immune factors.
- Difficulty ejaculating normally, as in the case of retrograde ejaculation (neurological problems, diabetes, etc.).
- Cases of cryopreserved samples from men who have had a vasectomy or have had chemotherapy or radiotherapy. These samples are very valuable as there is a limited quantity and ICSI allows their use to be optimised.
- Other factors: repeated failure following several cycles of IVF, a low number of oocytes obtained from follicular puncture, or when it is necessary to identify healthy embryos in the case of pre-implantation genetic diagnosis (PGD).